논문검색
Author Zheng Yun Cui, Ph.D.1, Jin Seok Ahn, M.D.2, Jee Yun Lee, M.D.2, Won Seog Kim, M.D.2, Ho Yeong Lim, M.D.2, Hyun Jung Jeon, V.M.D.3, Soo Won Suh, Ph.D.4, Jin Hoon Kim, M.S.4, Won Ho Kong, M.S.4, Ji Min Kang, B.S.4, Do Hyun Nam, M.D.5 and Keunchil Park, M.D.2
Place of duty 1Medical Nano Element Development Center, 2Division of Hematology and Oncology, Department of Medicine, 3Laboratory Animal Research Center, 4Department of Biomedical Engineering, 5Department of Neurosurgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Title Mouse Orthotopic Lung Cancer Model Induced by PC14PE6
Publicationinfo Cancer Res Treat 2006 Dec; 038(04): 234-239.
Key_word Orthotopic, PC14PE6, Lung cancer, Animal model
Full-Text
Abstract Purpose: This study was undertaken to investigate in detail the xenograft mouse orthotopic lung cancer model induced by PC14PE6 adenocarcinoma cells. Materials and Methods: Three cell doses (0.5⁓106; 1⁓106; 2⁓106) of PC14PE6 cells were injected into the lungs of male BALB/c nude mice by the intrathoracic injection method. The lung and other organs, including brain, liver, spleen, kidney, muscle, adrenal gland, and lymph node on knee, were removed and stained with H/E to detect the presence of tumor cells. Results: The reliable tumorigenicity time in the PC14PE6 adenocarcinoma cell-inoculated BALB/c nude mouse was 10 days after intrathoracic injection. The average lifespan of the three groups after inoculation was 14 days in the 2⁓106 cells inoculum group; 25 days in the 1⁓106 cells inoculum group; and 32 days in the 0.5⁓106 cells inoculum group. The PC14PE6 adenocarcinoma cells induced orthotopic lung cancer limited within the thorax. Conclusions: This orthotopic lung cancer model is an efficient cancer model with easy inoculation methods, rapid and high tumorigenicity, and simple monitoring methods for metastasis. (Cancer Res Treat. 2006;38:234-239)
출판정보 대한암학회지 2006 Dec; 038(04): 234-239.